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keratinocyte growth medium 3 (PromoCell)

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PromoCell keratinocyte growth medium 3
Keratinocyte Growth Medium 3, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/keratinocyte+growth+medium+3/Keratinocyte+Growth+Medium+3/pmc13084248-145-14-26
Average 94 stars, based on 1 article reviews

keratinocyte growth medium 3 - by Bioz Stars, 2026-11

94/100 stars

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Article Title: KLS-3021: Innovative oncolytic virotherapy for the treatment of advanced primary and metastatic cutaneous squamous cell carcinoma
Article Snippet: A-431 cells were cultured in minimum essential medium (MEM; Welgene, with Earle’s salts and L-glutamine) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% antibiotic-antimycotic (Gibco).

Article Title: FOXC1 Regulates Cytokine Signaling, Inflammatory Pathways, and Retinoid Metabolism to Maintain Limbal Epithelial Cell Homeostasis In Vitro.
Article Snippet: The resulting cell pellet was then resuspended in 800 μL of supplemented Keratinocyte Growth Medium 3 (KGM3; PromoCell GmbH, Heidelberg, Germany) containing 0.06 mM CaCl2 and the recommended supplement mixture.

Cell Culture:

Article Title: Patient-Derived Immortalized Limbal Epithelial Cells as In Vitro Models of Congenital Aniridia
Article Snippet: .. Biopsies obtained from both aniridia patients and healthy corneoscleral donor rim were subjected to overnight enzymatic digestion at 37 °C using collagenase A (Roche Pharma AG, Basel, Switzerland) and cultured in Keratinocyte Growth Medium 3 (KGM3; Cat. No. C-20021, PromoCell GmbH, Heidelberg, Germany), supplemented with the manufacturer’s supplement mix and CaCl 2 , as previously described [ ]. .. To separate limbal epithelial cells (pLEC) from limbal stromal cells (pLSC), the resulting cell suspension was filtered through a FlowmiTM cell strainer (40 μm pore size; SP Bel-Art Scienceware, Wayne, NJ, USA).

Article Title: Patient-Derived Immortalized Limbal Epithelial Cells as In Vitro Models of Congenital Aniridia.
Article Snippet: .. Biopsies obtained from both aniridia patients and healthy corneoscleral donor rim were subjected to overnight enzymatic digestion at 37 ◦C using collagenase A (Roche Pharma AG, Basel, Switzerland) and cultured in Keratinocyte Growth Medium 3 (KGM3; Cat. No. C-20021, PromoCell GmbH, Heidelberg, Germany), supplemented with the manufacturer’s supplement mix and CaCl2, as previously described [14]. .. To separate limbal epithelial cells (pLEC) from limbal stromal cells (pLSC), the resulting cell suspension was filtered through a FlowmiTM cell strainer (40 μm pore size; SP Bel-Art Scienceware, Wayne, NJ, USA).

Article Title: MicroRNA-378a-3p Modulates Inflammatory Responses of Keratinocytes to Atopic Dermatitis-Related Cytokines or Staphylococcus aureus
Article Snippet: .. Normal human epidermal keratinocytes (NHEKs; PromoCell, Heidelberg, Germany) were cultured in Keratinocyte Growth Medium 3 (KGM3; PromoCell) supplemented according to the manufacturer’s instructions. .. NHEKs were transfected with 60 nM miR-378a-3p mimic (Horizon Discovery, Cambridge, UK) or negative control (NC) mimic (Horizon Discovery) using cell-penetrating peptide PepFect14 (PF14) for 24 hours, as previously described ( ).

Incubation:

Article Title: In Vitro Analysis of Gene and Protein Expression in Primary Limbal Epithelial Cells Exposed to Differentiation-Inducing Medium
Article Snippet: .. These explants were incubated overnight at 37 °C in keratinocyte growth medium 3 (KGM3; PromoCell, Heidelberg, Germany) supplemented with 0.5 mg/mL collagenase A (Roche Pharma AG, Basel, Switzerland). .. On the following day, the digested limbal tissue fragments were pooled and passed through a 20 μm CellTrics ® filter (Sysmex, Norderstedt, Germany), thereby removing fibroblast contamination.

Article Title: miR-138-5p overexpression inhibits limbal epithelial cell proliferation and induces cell cycle arrest, in vitro.
Article Snippet: .. In brief, small pieces of limbal tissue (1.5 mm in diameter) were excised using a biopsy punch and incubated overnight at 37 ◦C in a 0.5 mg/ml collagenase A solution (Roche Pharma AG, Basel, Switzerland) prepared in keratinocyte growth medium 3 (KGM3; PromoCell, Heidelberg, Germany). .. After digestion, the tissue was gently dissociated by pipetting and filtered through a 20 μm CellTrics® filter (Sysmex Partec, Cat.-No. 04-004-2325, Goerlitz, Germany) to eliminate fibroblasts.

Article Title: In Vitro Analysis of Gene and Protein Expression in Primary Limbal Epithelial Cells Exposed to Differentiation-Inducing Medium
Article Snippet: .. These explants were incubated overnight at 37 ◦C in keratinocyte growth medium 3 (KGM3; PromoCell, Heidelberg, Germany) supplemented with 0.5 mg/mL collagenase A (Roche Pharma AG, Basel, Switzerland). .. On the following day, the digested limbal tissue fragments were pooled and passed through a 20 μm CellTrics® filter (Sysmex, Norderstedt, Germany), thereby removing fibroblast contamination.

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Keratinocytes cultured on modified collagen type IV show oxidative stress and reduced MMP levels. A Scatter plots show gene expression levels of IL1A and HMOX1 relative to B2M in keratinocytes cultured on HNE or OxPAPC modified collagen type IV. Primary keratinocytes from two donors (f38y, m27y) were grown in quadruplicates for up to 72 h, n = 8. B Heatmap of log 2 -transformed relative expression levels normalized to the corresponding control. Transcriptional levels of senescence, inflammation, and keratinocyte differentiation markers were assessed. Colors represent expression changes (blue = downregulation, red = upregulation). Statistical significance was determined by one-way ANOVA and is indicated by asterisks (* p < 0.05; ** p < 0.01; *** p < 0.005; **** p < 0.001) with p-values shown for z values > 1.

Journal: Redox Biology

Article Title: Modification of the dermal matrix by senescence associated lipids and its functional consequence

doi: 10.1016/j.redox.2026.104069

Figure Lengend Snippet: Keratinocytes cultured on modified collagen type IV show oxidative stress and reduced MMP levels. A Scatter plots show gene expression levels of IL1A and HMOX1 relative to B2M in keratinocytes cultured on HNE or OxPAPC modified collagen type IV. Primary keratinocytes from two donors (f38y, m27y) were grown in quadruplicates for up to 72 h, n = 8. B Heatmap of log 2 -transformed relative expression levels normalized to the corresponding control. Transcriptional levels of senescence, inflammation, and keratinocyte differentiation markers were assessed. Colors represent expression changes (blue = downregulation, red = upregulation). Statistical significance was determined by one-way ANOVA and is indicated by asterisks (* p < 0.05; ** p < 0.01; *** p < 0.005; **** p < 0.001) with p-values shown for z values > 1.

Article Snippet: After gelation at 37 °C in a humidified atmosphere for 3 h in the absence of CO 2 , gels were equilibrated in Keratinocyte Growth Medium (KGM2, PromoCell, Germany) and placed into an incubator with 5% CO 2 .

Techniques: Cell Culture, Modification, Gene Expression, Transformation Assay, Expressing, Control

Skin equivalents with modified collagen show disturbed differentiation and early senescence. A Hematoxylin & Eosin staining of skin equivalents. Collagen was modified, fibroblasts from different donors (f34y, f42y, m25y) were seeded into the matrix, and keratinocytes (f49y, f35y, f30y) on top; n = 6. SE were cultured at the air-liquid interface for 5 days to allow differentiation. B Quantification of nuclei in stratum corneum, indicating parakeratosis, n = 10. Bar graph shows mean ± SD; significant differences determined by one-way ANOVA (* p < 0.05; ** p < 0.01; *** p < 0.005; **** p < 0.001). C Representative images of SE stained with anti -KRT10, anti -KRT14, and Hoechst nuclear counterstaining. D Boxplot showing quantification of epidermal thickness, n = 4. Data are represented as median ± interquartile range; significance was determined by one-way ANOVA. E Protein expression of cell cycle inhibition marker P16 and housekeeping marker TUBULIN from the epidermis of the SE. F Bar chart showing quantification of P16 vol intensity normalized to TUBULIN with mean ± SD, n = 4, differences determined by one-way ANOVA. G Scatter plots showing expression levels of LMNB1, CDKN1A , and HSPA1A relative to B2M from epidermis and dermis of SE, n = 6. Asterisks represent significant differences determined by one-way ANOVA. H Confocal images of SE with anti -LMNB1 immunostaining and Hoechst nuclear counterstaining. I Boxplot showing quantification of LaminB1 mean signal intensity per pixel, measured in a virtual cross section through the epidermal cells, n = 4, significance determined by one-way ANOVA. J Confocal images of SE with anti -γH2AX immunostaining and Hoechst counterstaining. K Bar chart showing quantification of γH2AX positive nuclei by tissue cytometry analysis of the entire tissue section, mean ± SD, n = 3. Significant differences (* p < 0.05; ** p < 0.01) determined by Student's t - test. Scale bars: A = 50 μm; C = 100 μm; H, J = 20 μm.

Journal: Redox Biology

Article Title: Modification of the dermal matrix by senescence associated lipids and its functional consequence

doi: 10.1016/j.redox.2026.104069

Figure Lengend Snippet: Skin equivalents with modified collagen show disturbed differentiation and early senescence. A Hematoxylin & Eosin staining of skin equivalents. Collagen was modified, fibroblasts from different donors (f34y, f42y, m25y) were seeded into the matrix, and keratinocytes (f49y, f35y, f30y) on top; n = 6. SE were cultured at the air-liquid interface for 5 days to allow differentiation. B Quantification of nuclei in stratum corneum, indicating parakeratosis, n = 10. Bar graph shows mean ± SD; significant differences determined by one-way ANOVA (* p < 0.05; ** p < 0.01; *** p < 0.005; **** p < 0.001). C Representative images of SE stained with anti -KRT10, anti -KRT14, and Hoechst nuclear counterstaining. D Boxplot showing quantification of epidermal thickness, n = 4. Data are represented as median ± interquartile range; significance was determined by one-way ANOVA. E Protein expression of cell cycle inhibition marker P16 and housekeeping marker TUBULIN from the epidermis of the SE. F Bar chart showing quantification of P16 vol intensity normalized to TUBULIN with mean ± SD, n = 4, differences determined by one-way ANOVA. G Scatter plots showing expression levels of LMNB1, CDKN1A , and HSPA1A relative to B2M from epidermis and dermis of SE, n = 6. Asterisks represent significant differences determined by one-way ANOVA. H Confocal images of SE with anti -LMNB1 immunostaining and Hoechst nuclear counterstaining. I Boxplot showing quantification of LaminB1 mean signal intensity per pixel, measured in a virtual cross section through the epidermal cells, n = 4, significance determined by one-way ANOVA. J Confocal images of SE with anti -γH2AX immunostaining and Hoechst counterstaining. K Bar chart showing quantification of γH2AX positive nuclei by tissue cytometry analysis of the entire tissue section, mean ± SD, n = 3. Significant differences (* p < 0.05; ** p < 0.01) determined by Student's t - test. Scale bars: A = 50 μm; C = 100 μm; H, J = 20 μm.

Techniques: Modification, Staining, Cell Culture, Expressing, Inhibition, Marker, Immunostaining, Cytometry

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